Abigails Party Essay Example for Free

Abigails Party Essay My group and I are working on a play called the Birthday Party By Harold Pinter. The work is set in 1960s. The scene we doing is about a man, names Petey returning home from some sort of morning to job to a boarding house where his wife, whos called Meg and is the owner, fusses around him. We start to see that this routine is very basic and a sense of this what they do every day. As the scene goes on Stanley comes in and plays quite a grumpy teenager. I play the part of Petey. At the time of this plays setting, men had more of a dominant role in society and the women didnt work much and did more of the housework. This could mean that the male characters in plays around this time were more dominate and women did more housework and just like Meg, fussed around their husband as he was the only source of income, this could influence Meg as being fussy and concerning over Petey. The war would have been less than two decades ago so that may influence the characters in plays. Meg maybe fussing around he husband because he was a war-hero influencing her to very proud and caring of husband, or maybe Petey only has a morning job because he is slightly injured, this could influence his physical expression. This is different from the text that we have studied called Abigails Party because was set in the 1970s where women had a more dominant role than in the 1950s. The women were more out-spoken and more free to work have more social activities. In Abigails Party there was a lot more conflict and more fiery action between characters unlike in the Birthday Party it was Meg said a comment, which Petey may of thought was stupid, Petey just answered simply in a slow pace and showed no motivation to want to speak. In Abigails Party when a comment was made that one of the partners didnt like to hear they either fought about it or gave a sarcastic comment to show their annoyance whereas in the Birthday Party I, Petey, just gave short simple unmotivated answers to a persistent and fussing Meg. The two plays showed different ways of coping with situations as throughout the scene we performed Petey showed boredom and Meg carried on being quirky, fussy and questionable, in comparison to that in Abigails Party they handled situations in a more sarcastic, and argumentative. The two plays are humorous on different levels. The Birthday Party is funny because of we can see Peteys clear boredom of having this conversation and routine, which we can tell is a daily thing he goes through. Megs stupid comments just to speak and engage with Petey and in cases, to know out side life, is funny to us because the questions are stupid like when hes just got the corn flakes and she asks if they are nice and he hasnt even tasted them. However, the two plays have similarities. Pinter and Leigh are both known for naturalism. The scene on Birthday Party I performed was very natural and realistic nothing out of the ordinary happened it was very simple and plain but it was easy to relate to which made it effective and the birthday party as too naturalistic and perhaps more modern and in the effect of couple arguing and women playing more dominate roles. However, the Birthday Party as it goes on turns out to be more absurd and super-natural. Both plays also have fussy and inquisitive women. Meg is very questionable to Petey and wanting to know things, Bev. Tries to keep the gathering shes got alive and wanting people to pay attention to her likewise to Meg wanting Peteys attention. The historical and social setting is fairly similar as the man in Birthday Party does seem to have the dominant role as Meg does everything for him cooking, washing etc. Contrastingly to when Angie says something against Tony, like he is argumentative or wont do something, he takes action by shouting or grabbing her hand. Angie stops and doesnt protest showing men still had some dominant role in the 70s. We are presenting our play as the audience in a semi-circle in one end of the studio and us performing in the other half of the circle. This allows the audience to be the third wall, which is very in the style of Pinter and Leigh. This can give the audience more of a role and part, its very soap like, which is naturalistic, just as the plays studied, are intended to be. We are using different techniques and styles of performing such as giving each character more or less just one characteristic and not changing the pace much. For example when I play Petey I kept the same tone and tired motivation to tell the audience that Ive been doing this for a very long time. For Meg she kept her fussing and questionable ways and when Stanley comes down he was more mellow and got the attitude of Ive got my wrapped round my little finger. We used symbolism in our play, for example my paper. When I played Petey, the paper was my only bit of something new for that day, everything else was the same predictable self but my paper, which Im though out the scene reading or holding, gives me salivation from Meg and the predictable ways. We are keeping the style very naturalistic. This is different from the other play as the characters pace changed very much through the play. At one time they would be talking and the next minute there would be an argument to change the pace and the mood in which all the characters felt. You never felt the characters would stay for long at one place whereas Petey stayed in the chair all the time and kept his pace the same except it did change sometimes. The other text (Abigails party) influenced our performance by making Meg a little more dominate and persistent for Peteys attention.

Streptozotocin (STZ) Induced Diabetes Experiment

Streptozotocin (STZ) Induced Diabetes Experiment MATERIALS AND METHODS 3. MATERIALS AND METHODS 3.1 Animal Selection and Care STZ induced diabetes are commonly performed in Male Wistar Kyoto rats. In these models, male Wistar rats at 8 weeks of age (200–300 g) are selected from animal supply facility of SGPGIMS Lucknow, and house in a 12-h light, 12-h dark cycle environment, temperature 25 °C, with standard rat diet and water in metabolic cages for one week prior to STZ treatments. The study had clearance from Institute animal ethics committee. 3.2 Study Design Wistar Rat (Preparation and Validation of animal model of DN) 50 mg/kg STZ induced Diabetic Rat (n=3) Normal Rat (n=3) After 8th week rat are sacrificed for validation of Diabetic nephropathy 3.3 Induction and Validation of Diabetes by Streptozotocin There is a lack of appropriate animal model that could spontaneously develop DN. It has been a significant limitation to find out specific factors that is underlying this disease and also the development of new therapeutic methods or strategies to prevent progressive renal disease in diabetes (Morcelo A. Nobrega et. al. 2004). Most Sprague-Dawley (SD), Wistar-Kyoto (WKY) rats are used as model for performing STZ-induced diabetic nephropathy. Here, in these models, 8 weeks old male rats (200-250gm) are starved or kept on fasting for 18hrs and after that STZ was injected intraperitoneal (WKY-50mg/kg, SD-55mg/kg and SHR-45mg/kg) with sodium citrate buffer (1ml/kg) (Ma G et. al. 2004 and Cooper ME et. al. 1988). STZ given here intraperitoneally to the rats, however, it is a very less common procedure as intravenous injections are comparatively easy to perform in rats and give more reliable results. In addition to the STZ dosage required for inducing diabetes via an intraperitoneal route is relatively higher as compared to other route. After giving STZ, the rats should be given ample amount of drinking water with sucrose for 48 hrs (15g/L), to avoid early mortality of rats as stored insulin is released from damaged pancreatic Islets of Langerhans. After completion of 1 week of STZ induction, the rats must be assessed for hyperglycaemia and also those with fasting blood glucose of over 280 mg/dl (15 mmol/L), which is usually around 90%, and it should be included in the studies of diabetic nephropathy. In order to prevent subsequent development of ketone uria, subsequent injections of long-acting insulin (approx. 2-4 U/rat) should be given daily to maintain the blood glucose levels in a desirable range(300-600 mg/dl, 16-33 mmol/l) (Davis BJ et. al. 2003). Studies exploring the effects of treatment on the development of DN should not be underway until at least 3 weeks after STZ when the kidneys have improved from the acute mild nephrotoxic effects of STZ (Kraynak AR et . al. 1995) This drug i.e., STZ has been shown to induce a diabetic state in 72 hrs (3 days) as documented by examining tail blood samples using a Glucometer (Optimum Exceed) Diabetic animals and non-diabetic control group were kept in metabolic cages individually and separately and under feeding and metabolism control. Glucose in the blood of diabetic rats exceeded that of the non-diabetic control ones. Food consumption was measured in terms of (gm), water consumption was measured in terms of (ml) and urine volume was measured in terms of (ml) on a daily basis and glucose in blood serum were also measured, so that chemical diabetes was verified in rats injected with Streptozotocin. 3.4 Estimation of Creatinine Modified Jaffe’s method was used for colorimetric estimation of creatinine concentration in urine samples. Principle: Creatinine + picric acid Creatinine picrate (Yellow) (Orange) The resultant orange color is measured colorimetrically. However, the intensity of the resultant orange color is directly proportional to the concentration of creatinine in the sample. Method: Draw the Blood from a vein and then transferred into the vial. Centrifuge the blood for 10 minutes and Serum is obtained. Separate out the serum in different eppendrofs. The concentration of creatinine is calculated in the serum sample using the Jaffe’s method, as follows: In a clean dry test tube add 0.5 ml distilled water (blank) or serum (test), add 0.5 ml NaOH and then 0.5 ml picric acid. Mix all the contents of each tube. Left to stand for 15 minutes. The absorbance is measured at ÃŽ »max 500 nm. If a standard creatinine solution (0.55 mg/dl) has an absorbance value of 0.30, then the concentration of creatinine in the provided serum sample is calculated by using the following equation: Ctest = Cstd x 3.5 Estimation of Urinary Albumin Enzyme linked immunosorbent assay (ELISA) for detection of rat albumin in serum, plasma or urine. Other biological fluids that contain Rat Albumin, such as faeces or saliva, may be suitable samples. 3.5.1 Principle: The antigen present in urine sample are allowed to stick to a poly vinyl and then plate is washed to separate antigen and antibodies from remaining sample components. To this plate a corresponding second antibody is added to get fixed to the already adhered first antigen in the plate. To this added second antibody, an enzyme is also tagged is that, when a suitable substrate is added, the enzyme reacts with it to produce a colour. This colour produced is measurable as a function or quantity of antigen present in the urine sample and there by identified. 3.5.2 Chemical and material required: 96-well plate ELISA Coating Buffer ELISA Wash Solution ELISA Blocking Buffer Sample/Conjugate Diluent (ELISA Blocking Buffer + Tween 20) 10% Tween 20 Enzyme Substrate, TMB ELISA Stop Solution Additional Materials Required: Ultrapure water Precision pipettors, with disposable plastic tips Polypropylene, polyethylene or glass tubes to prepare standard and samples Containers to prepare buffers An aspiration device or an automated 96-well plate washer Disposable reagent reservoirs A standard microtiter plate reader for measuring absorbance at 450 nm 3.5.3 Procedure: Added 100  µl of diluted coating antibody to each well. Samples were run in duplicate. Incubated at room temperature (20-25 C) for 1 hour. Washed plate FIVE times. Added 200  µl of Blocking Solution to each well. Incubated at room temperature for 30 minutes. Washed plate FIVE times. Added 100  µl of standard or sample to well. Incubated at room temperature for 1 hour. Washed plate FIVE times. Added 100  µl of diluted HRP detection antibody to each well. Incubated at room temperature for 1 hour. Washed plate FIVE times. Added 100  µl of TMB Substrate Solution to each well. Developed the plate in the dark at room temperature for 15 minutes. Reaction was stopped by adding 100  µl of Stop Solution to each well. Absorbance was measured on a plate reader at 450 nm. 3.5.4 Precautions: Store all reagents at 2-8 ºC. Do not freeze reagents. All reagents must be at room temperature (20-25  ºC) before use. Vigorous plate washing is essential. Use new disposable pipette tips for each transfer to avoid cross-contamination. Minimize lag time between wash steps to ensure the plate does not become Completely dry during the assay. Avoid microbial contamination of reagents and equipment. Automated plate Washers can easily become contaminated thereby causing assay variability. Take care not to contaminate the TMB Solution. Do not expose TMB Substrate solution to glass, foils, or metal. If the solution is blue before use, do not use it 3.6 Oral Glucose Tolerance Test Rats are fasted overnight (12-16 hours) before the test, sedated rats, will be given 50 % dextrose (3ml/kg body weight) intraperitoneally. Whole blood will be collected from the tail vein at 0, 15, 30, 60, 120, and 180 min after the administered of glucose for the measurement of glucose with a glucometer. 3.6.1 Material Required: Glucometer and glucose strips Dextrose Gauge needles Timer and Pen Table of mice for record keeping of glucose values 3.6.2 Preparations before the test: Weigh the animals before the test. The animals are fasted overnight (approximately 16 hours: 5 pm to 9 am). Place each of the animals in a separate fresh cage with no food, but make sure they have water bottles. Make sure that there is no activity in the animal room before and while you are performing the test. Prepare the glucose solution the night before the test. 3.6.3 Procedure: After the overnight fast, blood glucose was determined (time 0) in a drop of blood as following: Rats were placed on top of the cage (let it hold onto the grid). The tail tip was pricked with a needle, wiped off the tail tip with gauze, and the next drop of blood was used for the determination of glucose with a glucometer. Glucose solution was given orally. Blood glucose was determined at 10, 20, 30, 60, 90 and 120 min after the administration of glucose. For the determination of glucose at each of these time points collected a drop of blood as following: wiped the cut end of the tail to break any blood clot that had formed and collected the next drop of blood. If the blood does not appear spontaneously â€Å"milk† the tail by holding the tail between your thumb and index finger and move along the tail from the base of the tail to the tip of the tail while applying gentle pressure. 3.7 Assessment of Renal Histopathological Injury 3.7.1 Tissue preparation for histology: After 8 weeks the rats were weighed, sacrificed out in accordance to the Institutional animal ethics committee by using suitable anaesthetic agent (Ketamine) and their kidneys were taken out. Left kidneys were perfusion fixed for Histopathological and IHC studies and right kidneys were freezed immediately for western blotting and RT-PCR. Preparation of paraffin blocks of kidney: Kidneys were preserved in Para- formaldehyde is taken out in a glass slab for sectioning. A two cross section of upper half of kidney was done with sterile blade for paraffin embedding. The whole process for preparation of paraffin blocks took two days. Day first: The Formalin fixed kidneys were picked up and place in plastic cassettes was sequentially dipped in alcohol for dehydration. The schedule is as followed: 50% Alcohol →1.5hrs 70%Alcohol →1 hrs. 80% Alcohol →1 hrs. 90% Alcohol →1hrs 100% Alcohol →1hrs (twice) The cassette containing the tissue was left overnight in 100% absolute alcohol. Day Second: On 2nd day we perform the following treatment to tissue containing cassette Cassette was removed from 100% alcohol and dipped for CHCl3 treatments Chloroform (A) 1.5 hours. Chloroform (B) 1.5 hours. Chloroform (C) 1.5 hours. The cassette was then kept in melted paraffin wax (at 58 °C 65 °C in water bath) following two changes of paraffin wax for proper blocking. The steel chocks are kept at the 65 °C electronic heater and the paraffin treated kidney in plastic cassettes are opened and place in pre-heated steel chocks together with melted paraffin wax and closed with cassette. Block was kept at room temperature to solidify the melted wax. The paraffin block containing tissue was sectioned with microtome. The block was fit properly in the Microtome machine 5 µ sections were cut. 3.8 Periodic Schiff’s Acid (PAS) Staining 3.8.1 Principle: PAS (Periodic Acid Schiff) is a method of staining used for the detection of polysaccharides i.e., glycogen and mucosubstances that is glycoprotein’s and glycolipids. PAS stain is a histochemical reaction. In the reaction, periodic acid oxidises vicinal diols in these sugar. It oxidized the aldehyde formed by carbon-carbon bonding that react with fuchsin-sulphurous acid and forms the magenta colour. This periodic acid exposes the glycogen to give a colouring product. The Schiff’s reagent is a very specific agent that only reacts with the carboxylic group compounds. Material required: Glass slides and cover slips Poly Lysine (Sigma Aldrich, USA) Xylene Graduated Alcohol (30%, 50%, 70%, 90% and 100%) Periodic acid Schiff Reagent Haematoxylin Acid alcohol (1% HCl in 70% alcohol) DPX mountant 3.8.3 Protocol for PAS Staining: Keep the Poly-Lysine coated slides on hot plate for 15-20 min. Dip in warm Xylene for 10 min twice. Pass the slide through graded alcohol 100% alcohol- (10 minutes) twice 90% alcohol (5 minutes) 70% alcohol (5 minutes) 50% alcohol (5 minutes) 30% alcohol (5 minutes) Dip in distilled water for 5 min. Place the slide containing section into 0.5 % Periodic acid for 5 minutes. Rinse with distilled water. Schiff Reagent was added for 10 min until deep magenta colour appear. Wash in running tap water for 5 min. Counter stain in haematoxylin Rinse with distilled water. If high stain come, then wash with acid alcohol (1% HCl+70% alcohol) Wash the slide in tap water. Dehydrate in alcohol, and dry the slides. After drying mount the slides by DPX mountant. Masson’s Trichrome Staining 3.9.1 Principle: MT Staining method is used to demonstrate the increase of collagen in diseases. This method of staining uses three dyes of contrasting colours for the selective staining of basic tissue components i.e., muscle, collagen fibers, fibrin, and erythrocytes. The general phenomenon of trichome staining is that smallest dye molecule colours or stains the less porous tissues. However, the penetration of dye of larger molecular size is depends on the expense of small molecules. Some others suggests that the acid dye firstly stained the tissue then the Biebrich Scarlet will binds with the acidophilic components of the tissue, after which when treated with the phosphor acids, the components that are less permeable will retain the red colour, because of the collagen this red colour is pulled out and at the same point of time causing a link with the collagen to bind with the aniline blue. . At the outset it must be made clear that the methods control how ionised acid dyes react with ionised basic tissues. 3.9.2 Material required: Bouin’s solution Xylene Graduated Alcohol (30%, 50%, 70%, 90% and 100%) Weigert’s iron hematoxylin solution Biebrich scarlet acid fusin phosphomolybedic-phosphotungustic acid solution Aniline blue solution 3.9.3 Protocol for MT Staining: Keep the Poly-Lysine coated slides on hot plate for 15-20 min. Dip in warm Xylene for 10 min twice. Pass the slide through graded alcohol 100% alcohol- (10 minutes) twice 90% alcohol (5 minutes) 70% alcohol (5 minutes) 50% alcohol (5 minutes) 30% alcohol (5 minutes) Dip in distilled water for 5 min. For formalin fixed tissue, re-fix in Bouin’s Solution for 30 minutes to 1 hr. at 56 ºC to improve the staining quality although this step is not necessary. Rinse in running tap water for 5-10 minutes to remove the yellow color. Stain in Weigert’s iron hematoxylin working solution for 10 minutes. Rinse in running warm tap water for 10 minutes. Wash in D/W Stain with Biebrich Scarlet Acid Fusin for 5 minutes. Differentiate in phosphophosphomolybedic-phosphotungustic acid solution for 10-15 minutes. Transfer the section directly (without rinse) to aniline blue solution and stain for 5-10 minutes. Rinse directly in D/W and differentiate in 1% Acetic acid solution for 2 minutes. Rinse slides, dehydrate through Alcohol Clean slides in xylene Mount with DPX mountant. NoteNuclei-Black, Muscle Fibre-Red, Collagen-Blue, Cytoplasm-Red Modified Immunohistochemistry 3.10.1 Principle: Immunohistochemistry (IHC) is the process whereby antibodies are used to detect proteins (antigens) in cells within a tissue section (for instance liver, pancreas or the heart). Immunohistochemistry exploits the principle that in biological tissues antibody binds to the specific antigens. This tool is used to localize specific antigens in tissue sections with labelled antibodies based on antigen-antibody interactions. This antigen-antibody interaction can be visualized in number of ways i.e., the immune reactive products can be visualized by a marker including fluorescent dyes, enzymes in general; radioactive elements or colloidal gold. This IHC technique is widely used by the researcher in basic research for understanding the differentially expressed proteins and for the localization and distribution of biomarkers in different- different parts of biological tissues. 3.10.2 Material required: Poly-Lysine coated slide. Xylene 1X- PBS buffer.(Sigma Aldrich Inc., USA) Graduated Alcohol (30%, 50%, 70%, 90% and 100%) DPX mountant for microscopy Acid alcohol (1% HCl in 70% alcohol) citrate buffer Sodium Borohydrate Hydrogen Peroxide Blocking solution Primary antibody Secondary antibody Streptovidin HRP 3.10.3 Protocol for Modified Immunohistochemistry: Cut the section of 3 to 5  µm. Warm the slide on hot plate (55 °c) for 30’. Dip the slide in warm Xylene twice for 10 minutes. Wash the slides three times in TBS or PBS for 5 minutes each. Dehydration using 100% Alcohol twice for 10 minutes each and 90, 80, 40, 50 and 30 percent for 5’ each in coupling jar. Wash thrice in TBS or PBS for 5 minutes each. Antigen retrieval -10 minutes for 98 °c in citrate buffer pH-6 (Note: 0.294 gm in 100 ml MQ with pH=6, 1 10mm citrate buffer) Here we are using pressure cooker for Antigen retrieval Cool the citrate Buffer slides. Give the Sodium Borohydrate treatment in coupling jar (To remove Background). (Dark Condition) (Note:- 1% NaBH4) Wash slides thrice in TBS or PBS for 5 minute each. Hydrogen Peroxide Blocking (3% H2O2 in Methanol or water –Dip the slides in it and keep it for 30’). Wash thrice in TBS or PBS for 5 minutes each. Blocking solution[0.3% Triton X and 5% sheep serum in 1xPBS or 1xTBS] For 2ml (6 µl Triton, 100  µl sheep serum, 19 of 94  µl (1xPBS or 1xTBS)) 14. Blocking for 30minutes at 37 °c in lab (some time 1hour also) Wash the slides thrice in TBS or PBS for 5’ each. Primary antibody in TBS or PBS solution, left overnight at 4 °c (Note: 1:200 dilution for fibronectin 1:500 dilution of Collagen IV). Wash the slides thrice in 1xTBS or 1xPBS Secondary antibody added on slide and keep it for 30’ to 1hour. But parafilm on it. (Note: 1:200 dilution) Wash the slide thrice in 1xTBSor 1XPBS for 5 minutes. Streptovidin HRP (1:200) in 1xTBS or 1xPBS for 20 minutes at Room temperature. Three wash in 1xTBS 5 minutes each. DAB (Till color in Brown) (15 minutes) 23.1xPBS (5minutes) wash 24. Counterstain for 30 seconds with Hematoxylene (Directly put on slide) 25. 1xTBS or PBS immediate wash 26. Dehydrate and dry and mount

The Aftermath Of The Scramble For Africa History Essay

The Aftermath Of The Scramble For Africa History Essay Scramble for Africa, an expression used to explain the frantic demanding of African region by half a dozen European countries that happened in most of Africa becoming part of Europes colonial kingdoms. Africa, in the symbolic allegory of royal chauvinism, was a ripe melon awaiting carving in the late nineteenth century. Those who scrambled quick achieved the biggest portions and the legal means to devour at their free time the sweet, juicy flesh. Stragglers grasped only small servings or flavorless sections; Italians, for instance, discovered only sweet dishes on their serving dish. In this crazy instant of royal atavismin Schumpeterian conditions, the aimless temperament to unlimited boundary extensionno one expected that a structure of states was being formed. Colonial rule, considered by its initiators to be eternal, afterward verified to be a sheer intermission in the broader removal of African history; however, the steel gridiron of regional division that colonialism enforced se ems enduring. (Harlow, 2002) Aftermath of scramble of Africa has the mainly significant issue of colonial heritage. It is the compulsory position of departure for breakdown analysis of African international associations. The country systemwhich is, international vectors despite, the basic structural foundation of the worldwide empiresucceeds the colonial division. A few African states have a significant pre-colonial identity (Morocco, Tunisia, Egypt, Ethiopia, Burundi, Rwanda, Madagascar, Swaziland, Lesotho, and Botswana), but nearly all are goods of the aggressive subordination of Africamajority between 1875 and 1900by seven European powers like Great Britain, France, Germany, Belgium, Portugal, Italy, and Spain. (Kobia, 2001) The scramble for Africa started with an effort by King Leopold II of Belgium desiring to attain power of the region of the Congo Basin. Pressure occurred between the British and the French, because of the British attaining additional power over Egypt, which was the country they once had combined power over the finances of. France was also contending with Italy in northern Africa, so tensions were tough all over the place. Germany felt stressed by the other European nations who were attaining power over regions on Africa (Neumann, 2002). Bismarck, who happened to be leader at the time, acknowledged power over three regions in eastern and western Africa, which created even more damage between European states. Since the power for African regions occurred very rapidly, the Berlin Conference was arrange to talk about the strategies of demanding realm in Africa to avoid any more harsh competitions. The motives for Scramble in Africa are described extensively as: Capitalists may have seen the light over slavery, but they still wanted to exploit the continent new legitimate trade would be encouraged. Explorers located vast reserves of raw materials, they plotted the course of trade routes, navigated rivers, and identified population centers which could be a market for manufactured goods from Europe. (Boddy-Evans, http://africanhistory.about.com/od/eracolonialism/a/ScrambleWhy.htm) After some adventurers looked deeper into the heart of Africa, the Europeans shortly comprehended how reasonably significant this region was, and how much they could take benefit from it. After the completion of servitude in Africa, Europeans desired to extend their kingdoms for industrialization and business to ensure the movement of supplies and services. Economic, communal, and political atmosphere in Europe produced an awareness of urgency amid viable countries to bet demands in and separation of the Dark Continent. In an attempt to produce some organization throughout the scramble, the Berlin Congress was held and European countries sliced up the African countries like a cake, every country got a piece of the land. The major countries comprised in the colonization of Africa contained France, England, Portugal, Germany, Great Britain and Denmark. Great Britain was the ultimate supremacy on earth at the time, and throughout the Scramble for Africa, it was the British who did most of the grasping. (Robinson, 1961) The five key grounds for the imperialism were supposed to be political, military interests, charitable and religious objectives, ideological, investigative, and finally, but most significantly, economic interests. One case of the monetary interest was the Industrial manufacture. Fabrication was attaining such extreme stages, Europeans concerned about over-production and finding customers for all the supplies in Europe. Their financial system mainly rested on trade, and because colonies could be added as a structure of royal power, it only furthered and extended trade. England had trade accords with nations in Africa sometime before the scramble in progress. These accords were fresh and allowed trade to occur without any disturbance. The responsibility and significance of Africa to England shortly changed because of royal rivalry among countries. Beneath the antagonistic strategies of Bismarck, Germany also deployed to obtain prime positions in Africa. Similarly, France was hoping to strengthen an empire by attaining new control over region and increasing areas of power. All the political forces, such as the rivalry with France, the demand to keep the Suez Canal, and the media well-versed public, forced England toward Africa. England incurred heavy losses from Africa. They lost thousands of soldiers to the combats, in which they were beaten quite a few times before finally adjoining some people who disliked them. (Pinfold, 2007) Besides, they mislaid thousands of dollars managing governments of nations such as Egypt. However Englands involvement di d benefit certain divisions of Africa through the charitable assistance and finally ending the slave trade. The results of the European takeover on Africans were substantial. In the short term, the Scramble noticeably guided to Africans defeat of power of their own relationships. While it also brought huge difficulty to the majority of Africans. In addition to the deaths caused by the victory itself, numerous Africans died as a consequence of disturbed standard of living and activity of people and animals among different diseased surroundings. Africas inhabitants did not initiate to recover from the destruction caused by the Scramble and its aftermath until healthy into the 20th century. In the enduring, the Scramble was component of a larger development of bringing non-Western peoples into the world economyin the majority instances as exporters of agricultural goods or minerals and importers of contrived or processed supplies. Colonial governments levied their African matters and utilized the revenues to advance the colonys infrastructure: building roads, bridges, and ports that associate d remote locales to the outer world. In the meantime, institutions to get better peoples lives, for example hospitals and schools, seemed more gradually. Colonial rule also brought fundamentals of Western culturefrom the French and English languages and Western political models to Coca-Cola and automobiles. It was in response to European regulation that Africans developed an awareness of patriotism that would assist them attain freedom in the mid of the 20th century. Imperialism influenced colonized states in numerous customs particularly economically, politically, and culturally. There were frequently numerous positive and negative results of imperialism on the colonies that were taken over. The civilization and religion of the colonized citizens was frequently destined to attempt to contain the citizen move in the approach of the westerners. In Africa, economically, Africans created very modest profit off of the supplies they produced. All of the assets went to the Europeans. Also, earlier than colonization, Africans traded inside the continent, but this exercise was finished once the westerners became engaged in their associations. So if anything, the colonial era, was one of monetary corruption, rather than economic growth. Colonization in Africa was somewhat beneficial to the African inhabitants. The value of life was enhanced by better infrastructure including hospitals, a sewage structure, and sanitary conveniences and there was also a boost in employment openings. Western discoveries for example, the steam engine and other equipment were introduced to Africa. Christianity and Islam were extended and so was western education. Colonialism created a modification in the social structure of Africans as it permitted mobilization among the categories. Social category was not verified by birth, but by ones achievement independently. Behind all of the optimistic social consequences, there were numerous pessimistic ones. A larger separation was produced among those who lived in urban regions and those in rural regions. Western education had created the barrier among these people even larger. Colonization permitted the wealthy, white Europeans to get the entire fertile and productive lands and also to dominate in trade in Africa. Although there were educational institutions build, they were inefficient in education the poor and needy. There were still very huge illiteracy levels. Also, there was no emphasis on technical or industrial education, which would have been further functional. Racism was prevalent throughout imperialism in Africa and ran rampant for people in the lower echelon of society. ( ) Politically, colonialism in Africa produced a superior level of peace and constancy than there ever was before. There were specific limitations in Africa, which was an excellent call in conditions of organization. There was also an established patriotism that extended all over the continent. On the other hand, a lot of the political modifications were negative. Because of limitations constructed by the westerners, numerous cultural and religious groups were ragged separately, which influenced the existences of the citizens on an individual level and formed numerous arguments (Kerr-Ritchie, 2007). The borders also did not make certain that natural resources were dispersed evenly, which would act as a difficulty since the economy of Africans was reliant on what they could collect from their ground. Africans lost their freedom and were fundamentally governed by the white colonial leaders, who also possessed approximately all of their property. For a long time, the people of Africa had l ost their right of autonomy. The significance of the colonial history in shaping modern African worldwide associations is thus ahead of argument. At the same time, the colonial system acted-paradoxically as a pessimistic point of allusion for the African performance of states. The authenticity of the first generation of African governments was rooted in the governments successby take-over or negotiationof self-government. The two superior united main beliefs of the pan-African action from its beginning have been resistance to both colonialism and racism, problems that were amalgamated on the African continent. The autonomous states that accumulated to generate the OAU in 1963 were separated on numerous questions of philosophy and understanding of objectivity; a convention behind the battle to complete the freedom of Africa from colonial occupation and governments of white racial supremacy. Within their own local area, self-governing states faced an obligation to separate themselves from their colonial history, t o render noticeable the new position. The superficial representative trappings of independenceflags and postage stampsmight help for an instance. Africanization of the situation apparatus might assist as well, though over time, the awareness could take place that the actual benefits of this transform accumulated above all to state employees. (Schneider, 2009) The obligation for separation from the colonial history was forced by mental as well as political and economic aspects. Mainly in sub-Saharan Africa, the colonial period brought a broad-front physical attack upon African culture that was far inclusive than alike practices in the Middle East and Asia. The colonial state of affairs, to borrow Georges Balandiers suggestive notion, was drenched with racism. (Carton, 2003) African culture was, for nearly all part, regarded as containing little worth, and its religious aspectouter the sectors in which Islam was well implantedwas aimed to pulling up through exhaustive Christian evangelical exertions, which were frequently state-supported. European languages displaced native ones for the majority of states; for the colonial matter, communal flexibility, obligatory mastering the idiom of the colonizer. In countless customs, colonial suppression in Africa brought not only political domination and economic utilization but also deep mental disgr ace. In the nationalist reply to colonialism, psychological subjects are widespread to a level exclusive in Third World anti-imperialist consideration. Frantz Fanon, the Martinique analyst who provided so influential a voice to the Algerian rebellion, was only the most expressive such spokesperson. Such policies as negritude and African personality were central elements in nationalist consideration, declaring the legitimacy and worth of African culture. This aspect of African nationalism gave a particular touching rim to the postcolonial mission for separation, as well as to the passion of African state response to racism and colonialism. A last heritage of the colonial system is the sequence of local disasters it has left in its wake, mainly in southern Africa and the Horn. In southern Africa, the basis of disagreement can be finally traced to the disastrous British fault of conveying authority to a solely white government in South Africa in 1910. Royal security calculus at the instance focused solely upon the associations among the English and Afrikaner communities. Practically the only dispensation to African interests was the preservation of colonial dominance over the Basutoland, Bechuanaland, and Swaziland protectorates. The terms of the Act of Union ultimately led to apartheid in South Africa. The year prior to the policy of paramount of local interests was announced for Kenya in 1924, Great Britain approved complete domestic autonomy to the white settlers in Southern Rhodesia (now Zimbabwe), a mistake that led to an expensive freedom war, prior to self-government stand upon equivalent rights for all Zimbabwean s was succeeded in 1980 (Cochran, 2000). When the moment of decolonization sounded somewhere else in Africa, South Africa, Rhodesia, and the Portuguese were in a situation to build a hard redoubt of white power, which left the subjugated no other options than the inert approval of enduring utilization or armed rebellion. The Scramble and its aftermath held huge sarcasm. While the take-over was going on, proceedings in Africa were of the utmost meaning in all over Europe. European rivalry for African area dominated captions, brought down governments, and approximately moved countries to war. For Europeans, the Scramble for Africa helped arrange the stage for World War I. Rivalry for African land boosted nationalist feelings and created pretentious awareness among Europeans that war was good for national character and not so taxing on financial plans and labour force. World War I quickly demolished these fantasies. Yet for Africa once the take-over was complete, Africa was mainly forgotten about and not acknowledged again until the movement for African freedom of the 1950s and 1960s. Thus, in different customs, the colonial heritage encroaches into post freedom African worldwide associations. More than half a century subsequent to the huge rush to freedom in 1960, the remnants of colonial shade still r emain. The intensity of the financial disaster and a broadening agreement that regional assimilation, which overpasses the old colonial separations is crucial to conquering them might guide to novelties in the state system that will start to rise above the colonial separation. The conclusion of apartheid in South Africa has shown hope of bringing harmony to a beleaguered area and authorizes movement beyond the harsh remainders of the colonial state of affairs. Even so, colonial inheritance at present continues to strongly form the African worldwide system.

J.B.Priestley’s play, An Inspector Calls Essay -- English Literature

By what means, and how successfully is suspense created and sustained in An Inspector Calls? An Inspector Calls Essay By what means, and how successfully is suspense created and sustained in ‘An Inspector Calls?’  · Show analytical and interpretative skills  · The effects of dramatic devices and structure  · Layers of meaning in language, ideas and themes  · The social and historical setting/context JB Priestley wrote ‘An Inspector Calls’ towards the end of World War 2. The play however, is set in 1912 at a wealthy, middle-class family home. The play is about a family celebrating an engagement, when an Inspector calls, and tells them of a young girl’s suicide. The play is always set in the family’s dining room, but it has precise stage directions, so each character must be sitting in a certain place. Suspense is used right through the play, usually at its peak at the end of an act. I think JB Priestley was trying to get a message across to us that we need to abolish the social class differences, and all be equal. The play is very good because you are always trying to find out who was responsible for the suicide, and you know that everybody fits in, but you don’t know when or how. As you find, or think you’ve found something out, the play takes a new dramatic turn, which definitely makes suspense the key factor. What makes the play even better is that the audience always knows a lot more than the characters do, and you are always wondering: â€Å"why haven’t they worked that out?† Suspense is very successfully created and sustained in ‘An Inspector Calls.’ When Act one begins, the mood is merry, and civilised in the dining room, at The Birlings’ house. The family are happy, excited and ... ...er this phone call means that yes everything was true, or no, it was a hoax. Then, it is used as a dramatic device again when it rings, and the local Inspector says he is coming round to question the Birlings about a young girl’s death. The play ends here. Now, we have found out the suspense is created and sustained very successfully in ‘An Inspector Calls’. With you always wondering whether the Inspector is real, and all the cliffhangers that are used, you are constantly left in suspense, even after the very unusual ending. You are wondering at the end whether this time it really is real, or is it another hoax? We will never know. Dramatic devices are a key element in this play, for example in Act 3; a telephone is the focal point. It can be little things like that. I hope this brings a good conclusion to a very good, interesting, even if strange play.

How to Masturbate :: essays research papers

Creative Masturbation Contraptions and Techniques: Cotton Balls Put glitter on the outside too to make it look pretty: Take a roll of toilet paper or paper towels (depending on what's more comfortable for you), put glue on the inside, then stuff it with cotton balls. You'll have yourself a nice sex toy, all you have to do is put your cock in and go for it. ------------------------------------------ Mummified Wanking Even better if you can find a nurse to help with the prep work: Stroke until you get hard then "lightly" (or as tight as you want) wrap an ace bandage around your cock. Round and round you go, until you wrap it all the way around. You now have a perfectly shaped "tube pussy". Once that is complete, gently remove your dick from the bandage, take a condom (or latex glove), anything rubber and smooth. Stick your dick into the hole where your cock was. Pull the edges out around the bandage and get a rubber band.. Put it around the edge to keep the latex item in place. After that, lube up your dick and have a blast! It's tight, just like a real cunt. For best results, put a pillow under and over the bandage; sandwich it while you fuck it. Also, it's a no-mess situation.. Fuck the shit out of it, cum in it. then just throw the condom or latex item away.. The bandage will keep it's form for future use. ------------------------------------------ Balloon magic Nice: Get about four or five balloons. Fill them with warm, not hot, water. Tie the ends so water will not squeeze out. Take a pillow; roll it up like a tube and tie a string around it or even use your belt. Push the warm water balloons into the opening of the pillow tube. Your fleshy warm vagina is almost ready. Take a small plastic kitchen trash can bag. Push it in between the balloons in the pillow. Put some lubricant of your choice inside the plastic bag that is being tightly squeezed by the balloons. Get your dick ready. Put the pillow on bed. Put your dick inside this plastic vagina. You will feel the warmth, slipperiness and tightness you only dreamed of. ------------------------------------------ Baby Bottle Liner You first might want to throw away the box with the picture of the baby on it: They make 'drop-in' baby bottle liners.. get the 8 ounce size.. comes 20 or so to a box.

Populism DBQ

In the late nineteenth century, around 1880-1900, many farmers were experiencing problems and threats to their way of life. The valid complaints of the farmers dealt with the money supply system in America and the large railroad companies. In 1892, the platform for the Populist Party was laid down. In this platform it is stated that â€Å"the national power to create money is appropriated to enrich bondholders †¦ thereby adding millions to the burdens of the people.†This is discussing the demonetization of silver and the negative effect it has on the common people, such as farmers. Later on in the platform is it also discussed that silver has had widespread acceptance as a coin for a very long time and by demonetizing it to increase the purchasing power of gold, the results are several negative consequences which will eventually lead to â€Å"terrible convulsions, the destruction of civilization, or the establishment of an absolute despotism.†This unhappiness of fa rmers regarding the money system in the United States is also shown in a political cartoon from The Farmers Voice, a Chicago newspaper in the late 1880's or early 1890's. The cartoon entitled â€Å"The Eastern Master and His Western Slaves† depicts farmers as slaves to the wealthy eastern businessmen. It is representing the exploitation of the farmers and shows yet another of their economic struggles; the mortgages they bore on their farms.Further evidence that supports and validates the farmers' complaints about the current economic situation is found in William McKinley's acceptance speech given in Canton, Ohio on August 26, 1896. In his speech, McKinley said that even though free silver â€Å"would not make farming less laborious and more profitable.. † farmers and laborers are the ones who suffer the greatest as a result of the cheap money. â€Å"They are the first to feel its bad effects and the last to recover from them†¦ †. The belief that silver is the solution of the problems for farmers is opposed in J.Laurence Laughlin's â€Å"Causes of Agricultural Unrest† article in the November, 1896 issue of Atlicantic Monthly. Laughlin describes that the increase in supply without an increase of demand led farmers to believe that silver can solve their issues by his saying, â€Å"the sudden enlargement of the supply without any corresponding increase of demand produced that alarming fall in the price of wheat which has been made the farmer's excuse for thinking that silver is the magic panacea for all his ills†¦Ã¢â‚¬ He then goes on to describe that farmers have simply pushed the blame on the scarcity of gold as opposed to realizing the actual cause is their own overproduction of wheat. The effects of the different acts and laws regarding money supply is shown in the United States government data from 1961 depicting the population of the country along side the money in circulation from the year 1865 through 1895. This dat a shows that from 1865 through 1885, the population was increasing, however the amount of money in circulation was decreasing rapidly.This suggests that the effects of the acts and laws regarding money were resulting in the deflation of of the currency. against the railroad companies is credible because during this time period the government showed enormous favoritism towards large businesses even though the railroads were monopolies. Further discontent with monopolies is expressed in A Call to Action: An Interpretation of the Great Uprising. Its Source and Causes by James B.Weaver, a Populist candidate for president in the election of 1892. Weaver described that trusts and monopolies use â€Å"threats, intimidation, bribery, fraud, wreck, and pillage† to â€Å"impoverish the producer, drive him to a single market, reduce the price of every class of labor connected with the trade, throw out of employment large numbers of people †¦ , and finally †¦ they increase the price to the consumer†¦ †.The farmers and laborers of the late nineteenth century faced two main problems; money supply and large businesses such as railroads. These issues resulted in a variety of complaints from the agriculturists, however the grievances did prove to be valid based on the evidence previously presented. The farmers were struggling to survive off of the small profit they received, and they suffered even further when large monopolies and railroad companies took actions that dwindled their profits further.

Erp as Software as a Service (Saas) for Smes

ERP as Software as a Service *(*SaaS*)* for SMEs Table of Contents Page Abstract The main barriers for implementing ERP systems in SMEs were constraints in terms of resources and information, implementation time escalations, poorly defined organisational structures, weak formalisation of procedures or processes, unclear and also confused understanding amongst the employees and management. There are various methods to overcome the barriers of implementing ERP solutions in SMEs. This include coming up with a realistic value-adding processes required to be incorporated in the proposed ERP system, providing effective education on ERP systems, making sure as a vendors, they do not just provide lip service and to involve everyone in the whole system’s lifecycle. Software-as-a-service (SaaS) is an online delivery of softwares (Dubey & Wagle 2007). The model is uncomplicated and appealing wherein companies signs up to the use the solution which is hosted by the vendors that sells and/or develops the software rather than spending large sums on an application such as ERP and Customer Relationship Management (CRM) and installing this software on individual machines (Dubey & Wagle 2007; Banham 2008). All of the major software vendors are now offering its solution on SaaS model basis – SAP, Microsoft, IBM, Oracle and Salesforce. com are all staking their claims. Introduction SME Definition â€Å"companies with less than 250 employees, total assets under 27 million Euros,_ independent privately held_ and _ _owner-managers or their families manage the company personally (Loecher 2000; Sogorb 2002)†. _ Why ERP vendors are focusing on SME’s Barriers to implementation of ERP systems in SME’s It was found that the main barriers for implementing ERP systems in SMEs were: constraints in terms of resources and inf ormation, implementation time escalations, poorly defined organisational framework, informal and also non-orderly procedures or processes, unclear and also confused understanding amongst the employees and management. Each of these factors is now discussed in turn. _Figure 1: ERP implementation issues (source: Bernroider & Leseure 2005)_ How ERP vendors can overcome these barriers SaaS Technology Some other advantages of working with SaaS models include: low introduction and subscription costs and faster initiating speed (Kaplan 2005; Torbacki 2008), elimination of license and upgrade costs (Torbacki 2008), predictability of costs (Torbacki 2008), ow TCO costs (Total Cost of Ownership) due to lack of necessity of major investments in servers, databases and maintenance (Torbacki 2008), generating a greater ROI than traditional software packages (Kaplan 2005) no requirement of installing any software on a workstation (Torbacki 2008), online access from any location with an Internet connection (Torbacki 2008), continuous opportunity to the newest software versions and business technologies (Torbacki 2008), customers no longer require the large support staff for applications (Bontis & Chung 2000; Kapl an 2005) {draw:frame} Figure 2: Types of SaaS used or considering of using (source: Kaplan 2009)_ An example of one vendor’s SaaS offering Nationwide Express Farwest Steel Corporation SaaS alternatives have become more pervasive and observed fast development, and their cost-effectiveness, operational reliability become more obvious and there are a growing number of enterprises of all sizes that are generating performance improvements and measurable cost savings as a result of adopting SaaS. For SaaS vendors, they must focus on make possible easier deployments to avoid the need for pricey consultation support. Also they should truly deliver lower total cost of ownership (TCO) and offer more robust strategies for integration that acknowledges the current volatile environments and also in the near future. Finally, the SaaS solutions offered must be simpler, more agile, more robust, and more intuitive and worthy of all the investments allocated. References Banham, R 2008, ‘Up and Away – Companies are feeling a little less trapped by spreadsheets these days’, CFO Magazine, viewed 19 October 2009, Bernroider, E. W. N & Leseure, M. J 2005, ‘Enterprise resource planning (ERP) diffusion and characteristics according to the system’s lifecycle: A comparative view of small-to-medium sized and large enterprises’, Working Papers on Information Processing and Information Management, Institute of Information Processing and Information Management, Vienna University of Economics and Business Administration. http://epub. wu. ac. at/dyn/virlib/wp/eng/mediate/epub-wu-01_84b. pdf? ID=epub-wu-01_84b Bontis, N & Chung, H 2000, ‘The evolution of software pricing: from box licenses to application service provider models’, Internet Research: Electronic Networking Applications and Policy, Vol. 10, No. 3, pp. 246-255. Christodoulou, M 2009, ‘How big is an SME? Confusion over size could complicate international rules’, Accountancy Age, pp. 7 http://www. accountancyage. com/accountancyage/analysis/2246584/big-sme-confusion-size Deep, A, Guttridge, P, Dani, S & Burns, N 2007, ‘Investigating factors affecting ERP selection in made-to-order SME sector’, Journal of Manufacturing Technology Management, Vol. 9, No. 4, pp. 430-446. Dubey, A, Wagle D 2007, ‘Delivering Software as a Service’, The McKinsey Quarterly. http://www. mckinsey. de/downloads/publikation/mck_on_bt/2007/mobt_12_Delivering_Software_as_a_Service. pdf European Commission 2009, ‘SME Definition’, viewed 17 October 2009, Gartner 2009, Gartner Sur vey Shows Many Users are underwhelmed by Their Experiences of SaaS, viewed 17 October 2009, Harindranath, G, Dyerson, R & Barnes, D 2008, ‘ICT Adoption and Use in UK SMEs: a Failure of Initiatives? , _The _Electronic Journal Information Systems Evaluation, Volume 11, Issue 2, pp. 91 – 96. IBM 2009a, Software as a Service, viewed 17 October 2009, IBM 2009b, ‘Managed server services’, viewed 17 October 2009, IBM 2009c, Software as a Service from IBM and Innovative Computing Corp. delivers speedy customer service for Nationwide Express, IBM Software as a Service, viewed 18 October 2009, Jacobson, S, Shepherd, J, D’Aquila, M & Carter, K 2007, ‘The ERP Market Sizing Report, 2006–2011’, AMR